Composite

Part:BBa_K1615058:Design

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-09-02)


Heroin esterase fused to CBDcenA+Linker in RFC25 driven by LacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1665
    Illegal BamHI site found at 2421
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
    Illegal NgoMIV site found at 2623
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Fusion was performed using RFC10 standard due to illegal NgoMIV site in f1 origin.


Source

Heroin Esterase from Rhodococcus erythropolis. CBDcenA from Cellulomonas fimi. Lac expression cassette from iGEM10_Washington.

References